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Cystatin A Recombinant Antibody, Cy3 Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
Overview
Catalog # bsm-62286r-cy3-100uL
Product Name Cystatin A Recombinant Antibody, Cy3 Conjugated
Applications
WB FCM
Reactivity Human
Predicted Reactivity Mouse, Rat
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Cystatin A
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1475
Swiss Prot P01040
Subcellular location Cytoplasm
Synonyms AREI; cstA; Cystatin A; Cystatin AS; Stefin A; STF1; STFA.
Background This is an intracellular thiol proteinase inhibitor. Has an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100

Frequently asked questions

Which applications is Cystatin A Recombinant Antibody, Cy3 Conjugated (bsm-62286R-Cy3) validated for, and at what dilution?

bsm-62286R-Cy3 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Cystatin A Recombinant Antibody, Cy3 Conjugated (bsm-62286R-Cy3) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.

How should bsm-62286R-Cy3 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Cystatin A Recombinant Antibody, Cy3 Conjugated (bsm-62286R-Cy3)?

KLH conjugated synthetic peptide derived from human Cystatin A

What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-62286R-Cy3)?

Cy3 on bsm-62286R-Cy3: excitation / emission 512,550nm/570,615nm.