TCF4 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62320r-cy3 |
| Product Name | TCF4 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TCF4 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6925 |
| Swiss Prot | P15884 |
| Synonyms | E2-2; ITF2; PTHS; SEF2; ITF-2; SEF-2; TCF-4; SEF2-1; SEF2-1A; SEF2-1B; bHLHb19. |
| Background | Transcription factor that binds to the immunoglobulin enhancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:50-200 |
Frequently asked questions
Which applications is TCF4 Recombinant Antibody, Cy3 Conjugated (bsm-62320R-Cy3) validated for, and at what dilution?
bsm-62320R-Cy3 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TCF4 Recombinant Antibody, Cy3 Conjugated (bsm-62320R-Cy3) react with?
Tested reactivity: Human.
How should bsm-62320R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TCF4 Recombinant Antibody, Cy3 Conjugated (bsm-62320R-Cy3)?
KLH conjugated synthetic peptide derived from human TCF4
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-62320R-Cy3)?
Cy3 on bsm-62320R-Cy3: excitation / emission 512,550nm/570,615nm.