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WNT 5B Recombinant Antibody, Cy5 Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human
Overview
Catalog # bsm-62330r-cy5
Product Name WNT 5B Recombinant Antibody, Cy5 Conjugated
Applications
WB FCM
Reactivity Human
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Wnt5b
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 81029
Swiss Prot Q9H1J7
Subcellular location Secreted
Synonyms Wnt5b
Background Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100

Frequently asked questions

Which applications is WNT 5B Recombinant Antibody, Cy5 Conjugated (bsm-62330R-Cy5) validated for, and at what dilution?

bsm-62330R-Cy5 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does WNT 5B Recombinant Antibody, Cy5 Conjugated (bsm-62330R-Cy5) react with?

Tested reactivity: Human.

How should bsm-62330R-Cy5 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise WNT 5B Recombinant Antibody, Cy5 Conjugated (bsm-62330R-Cy5)?

KLH conjugated synthetic peptide derived from human Wnt5b

What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-62330R-Cy5)?

Cy5 on bsm-62330R-Cy5: excitation / emission 625,650nm/670nm.