HMGN2/HMG17 Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62335r-cy5 |
| Product Name | HMGN2/HMG17 Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HMGN2 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3151 |
| Swiss Prot | P05204 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | HMG17; HMGN2. |
| Background | Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is HMGN2/HMG17 Recombinant Antibody, Cy5 Conjugated (bsm-62335R-Cy5) validated for, and at what dilution?
bsm-62335R-Cy5 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HMGN2/HMG17 Recombinant Antibody, Cy5 Conjugated (bsm-62335R-Cy5) react with?
Tested reactivity: Human.
How should bsm-62335R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HMGN2/HMG17 Recombinant Antibody, Cy5 Conjugated (bsm-62335R-Cy5)?
KLH conjugated synthetic peptide derived from human HMGN2
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-62335R-Cy5)?
Cy5 on bsm-62335R-Cy5: excitation / emission 625,650nm/670nm.