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eIF3B Recombinant Antibody, AbBy Fluor-647 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-62337r-bf647
Product Name eIF3B Recombinant Antibody, AbBy Fluor-647 Conjugated
Applications
WB FCM IF(IHC-P) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation AbBy Fluor-647
Host Rabbit
Source KLH conjugated synthetic peptide derived from human eIF3B
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 8662
Swiss Prot P55884
Subcellular location Cytoplasm
Synonyms eIF3b; EIF3S9; hPrt1; PRT1.
Background Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis.
Application Dilution
WB 1:500-3000
FCM FCM1:50-100
IF(IHC-P) IFIHC-P1:100-500
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is eIF3B Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-62337R-BF647) validated for, and at what dilution?

bsm-62337R-BF647 is validated for WB(WB) (1:500-3000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does eIF3B Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-62337R-BF647) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-62337R-BF647 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise eIF3B Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-62337R-BF647)?

KLH conjugated synthetic peptide derived from human eIF3B

What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-62337R-BF647)?

AbBy Fluor-647 on bsm-62337R-BF647: excitation / emission 650nm/665nm.