Nm23-H2/NME2 Recombinant Antibody

$The 293T (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Nm23-H2\/NME2 antibody (bsm-62342R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The 293T (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Nm23-H2\/NME2 antibody (bsm-62342R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

$Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nm23-H2\/NME2 Monoclonal Antibody, Unconjugated (bsm-62342R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nm23-H2\/NME2 Monoclonal Antibody, Unconjugated (bsm-62342R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nm23-H2\/NME2 Monoclonal Antibody, Unconjugated (bsm-62342R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nm23-H2\/NME2 Monoclonal Antibody, Unconjugated (bsm-62342R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nm23-H2\/NME2 Monoclonal Antibody, Unconjugated (bsm-62342R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Nm23-H2\/NME2 Monoclonal Antibody, Unconjugated (bsm-62342R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$25 ug total protein per lane of various lysates (see on figure) probed with Nm23-H2\/NME2 monoclonal antibody, unconjugated (bsm-62342R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Nm23-H2\/NME2 monoclonal antibody, unconjugated (bsm-62342R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IHC-P
IHC-F
IF
Flow-Cyt
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-62342r
Product Name	Nm23-H2/NME2 Recombinant Antibody
Applications	WB IHC-P IHC-F IF FLOW-CYT ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human NDK B
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4831
Swiss Prot	P22392
Subcellular location	Cytoplasm, Nucleus
Synonyms	C myc purine binding transcription factor PUF; MGC2212; NDKB; NDP kinase B; NDPKB; NM23B; Nucleoside diphosphate kinase B; PUF.
Background	NM23 nucleoside diphosphate kinase A. Major role in the synthesis of nucleoside triphosphates other than ATP. Holoenzyme is a hexamer of the A and B subunits. Mutations have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been described.
Application Dilution
WB	1:300-5000
IHC-P	FCM1:20-100
IHC-F	IHC-P1:200-400
IF	IHC-F1:100-500
Flow-Cyt	IFICC1:50-200
ICC/IF	IF