MonoMethyl-Histone H2B type 2E (Lys117) Recombinant Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62344r-bf405 |
| Product Name | MonoMethyl-Histone H2B type 2E (Lys117) Recombinant Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Histone H2B type 2E around the methylation site of K117 |
| Modification Site | Lys117 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified Polyclonal A |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8349 |
| Swiss Prot | Q16778 |
| Synonyms | Histone H2B |
| Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is MonoMethyl-Histone H2B type 2E (Lys117) Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-62344R-BF405) validated for, and at what dilution?
bsm-62344R-BF405 is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MonoMethyl-Histone H2B type 2E (Lys117) Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-62344R-BF405) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62344R-BF405 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MonoMethyl-Histone H2B type 2E (Lys117) Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-62344R-BF405)?
KLH conjugated synthetic peptide derived from human Histone H2B type 2E around the methylation site of K117
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bsm-62344R-BF405)?
AbBy Fluor-405 on bsm-62344R-BF405: excitation / emission 401nm/421nm.