LCAT Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-62349r-cy3 |
| Product Name | LCAT Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human LCAT |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3931 |
| Swiss Prot | P04180 |
| Subcellular location | Secreted |
| Synonyms | LCAT |
| Background | Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is LCAT Recombinant Antibody, Cy3 Conjugated (bsm-62349R-Cy3) validated for, and at what dilution?
bsm-62349R-Cy3 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LCAT Recombinant Antibody, Cy3 Conjugated (bsm-62349R-Cy3) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should bsm-62349R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise LCAT Recombinant Antibody, Cy3 Conjugated (bsm-62349R-Cy3)?
KLH conjugated synthetic peptide derived from human LCAT
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-62349R-Cy3)?
Cy3 on bsm-62349R-Cy3: excitation / emission 512,550nm/570,615nm.