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LCAT Recombinant Antibody, Cy5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
Overview
Catalog # bsm-62349r-cy5
Product Name LCAT Recombinant Antibody, Cy5 Conjugated
Applications
WB IF(IHC-P) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human LCAT
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3931
Swiss Prot P04180
Subcellular location Secreted
Synonyms LCAT
Background Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs).
Application Dilution
WB 1:500-2000
IF(IHC-P) IFIHC-P1:100-500
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is LCAT Recombinant Antibody, Cy5 Conjugated (bsm-62349R-Cy5) validated for, and at what dilution?

bsm-62349R-Cy5 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does LCAT Recombinant Antibody, Cy5 Conjugated (bsm-62349R-Cy5) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.

How should bsm-62349R-Cy5 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise LCAT Recombinant Antibody, Cy5 Conjugated (bsm-62349R-Cy5)?

KLH conjugated synthetic peptide derived from human LCAT

What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-62349R-Cy5)?

Cy5 on bsm-62349R-Cy5: excitation / emission 625,650nm/670nm.