NLK Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with NLK monoclonal antibody, unconjugated (bsm-62399R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with NLK monoclonal antibody, unconjugated (bsm-62399R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human

Overview
Catalog #	bsm-62399r
Product Name	NLK Recombinant Antibody
Applications	WB, FCM
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human NLK
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	51701
Swiss Prot	Q9UBE8
Subcellular location	Cytoplasm, Secreted
Synonyms	LAK1; Nemo like kinase; Nlk.
Background	Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination.Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2.Negative regulator of the canonical Wnt/beta-catenin signaling pathway.
Application Dilution
WB	1:500-2000
FCM	FCM1:50-100