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NLK Recombinant Antibody, AbBy Fluor-555 Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human
Overview
Catalog # bsm-62399r-bf555
Product Name NLK Recombinant Antibody, AbBy Fluor-555 Conjugated
Applications
WB FCM
Reactivity Human
Specifications
Conjugation AbBy Fluor-555
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NLK
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 51701
Swiss Prot Q9UBE8
Subcellular location Cytoplasm, Secreted
Synonyms LAK1; Nemo like kinase; Nlk.
Background Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination.Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2.Negative regulator of the canonical Wnt/beta-catenin signaling pathway.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100

Frequently asked questions

Which applications is NLK Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-62399R-BF555) validated for, and at what dilution?

bsm-62399R-BF555 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NLK Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-62399R-BF555) react with?

Tested reactivity: Human.

How should bsm-62399R-BF555 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NLK Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-62399R-BF555)?

KLH conjugated synthetic peptide derived from human NLK

What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bsm-62399R-BF555)?

AbBy Fluor-555 on bsm-62399R-BF555: excitation / emission 553nm/568nm.