NLK Recombinant Antibody, Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62399r-cy7 |
| Product Name | NLK Recombinant Antibody, Cy7 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NLK |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 51701 |
| Swiss Prot | Q9UBE8 |
| Subcellular location | Cytoplasm, Secreted |
| Synonyms | LAK1; Nemo like kinase; Nlk. |
| Background | Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination.Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2.Negative regulator of the canonical Wnt/beta-catenin signaling pathway. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
Frequently asked questions
Which applications is NLK Recombinant Antibody, Cy7 Conjugated (bsm-62399R-Cy7) validated for, and at what dilution?
bsm-62399R-Cy7 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NLK Recombinant Antibody, Cy7 Conjugated (bsm-62399R-Cy7) react with?
Tested reactivity: Human.
How should bsm-62399R-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NLK Recombinant Antibody, Cy7 Conjugated (bsm-62399R-Cy7)?
KLH conjugated synthetic peptide derived from human NLK
What are the excitation and emission wavelengths of the Cy7 conjugate (bsm-62399R-Cy7)?
Cy7 on bsm-62399R-Cy7: excitation / emission 743nm/767nm.