PAG1 Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62406r-rbitc-100uL |
| Product Name | PAG1 Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PAG1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 55824 |
| Swiss Prot | Q9NWQ8 |
| Subcellular location | Cell membrane |
| Synonyms | CBP; Csk binding protein; PAG; PAG1. |
| Background | Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is PAG1 Recombinant Antibody, RBITC Conjugated (bsm-62406R-RBITC) validated for, and at what dilution?
bsm-62406R-RBITC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PAG1 Recombinant Antibody, RBITC Conjugated (bsm-62406R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62406R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PAG1 Recombinant Antibody, RBITC Conjugated (bsm-62406R-RBITC)?
KLH conjugated synthetic peptide derived from human PAG1
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-62406R-RBITC)?
RBITC on bsm-62406R-RBITC: excitation / emission 570nm/595nm.