Crotonyl-Histone H2B type 2E (Lys12) Recombinant Monoclonal Antibody, APC-Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62430r-apc-cy5 |
| Product Name | Crotonyl-Histone H2B type 2E (Lys12) Recombinant Monoclonal Antibody, APC-Cy5 Conjugated |
| Applications |
WB
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Histone H2B type 2E around the crotonylation site of K12 |
| Modification Site | Lys12 |
| Clonality | Monoclonal |
| Clone # | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8349 |
| Swiss Prot | Q16778 |
| Synonyms | Histone H2B type 1-H; Histone H2B.j; H2B/j; HIST1H2BH; H2BFJ. |
| Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Crotonyl-Histone H2B type 2E (Lys12) Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-62430R-APC-Cy5) validated for, and at what dilution?
bsm-62430R-APC-Cy5 is validated for WB (1:300-5000); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Crotonyl-Histone H2B type 2E (Lys12) Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-62430R-APC-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62430R-APC-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Crotonyl-Histone H2B type 2E (Lys12) Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-62430R-APC-Cy5)?
KLH conjugated synthetic peptide derived from human Histone H2B type 2E around the crotonylation site of K12
What are the excitation and emission wavelengths of the APC-Cy5 conjugate (bsm-62430R-APC-Cy5)?
APC-Cy5 on bsm-62430R-APC-Cy5: excitation / emission 650nm/670nm.