Mono/Di/TriMethyl-Histone H3.1 (Lys15) Recombinant Monoclonal Antibody, PE-Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62431r-pe-cy3 |
| Product Name | Mono/Di/TriMethyl-Histone H3.1 (Lys15) Recombinant Monoclonal Antibody, PE-Cy3 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | PE-Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Histone H3.1 around the methylation site of K15 |
| Modification Site | Lys15 |
| Clonality | Monoclonal |
| Clone # | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8350 |
| Swiss Prot | P68431 |
| Subcellular location | Nucleus |
| Synonyms | H3 histone; HIST1H3A; Histone cluster 1, H3a;H3/l; HIST3H3; H3K14me1; H3K14me2; H3K14me3. |
| Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Mono/Di/TriMethyl-Histone H3.1 (Lys15) Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-62431R-PE-Cy3) validated for, and at what dilution?
bsm-62431R-PE-Cy3 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Mono/Di/TriMethyl-Histone H3.1 (Lys15) Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-62431R-PE-Cy3) react with?
Tested reactivity: Human, Mouse.
How should bsm-62431R-PE-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Mono/Di/TriMethyl-Histone H3.1 (Lys15) Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-62431R-PE-Cy3)?
KLH conjugated synthetic peptide derived from human Histone H3.1 around the methylation site of K15
What are the excitation and emission wavelengths of the PE-Cy3 conjugate (bsm-62431R-PE-Cy3)?
PE-Cy3 on bsm-62431R-PE-Cy3: excitation / emission 488nmn/578nm.