Aconitase 1 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with Aconitase 1 monoclonal antibody, unconjugated (bsm-62433R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Aconitase 1 monoclonal antibody, unconjugated (bsm-62433R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-62433r
Product Name	Aconitase 1 Recombinant Antibody
Applications	WB IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Aconitase 1
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	48
Swiss Prot	P21399
Subcellular location	Cytoplasm
Synonyms	ACO 1; Aconitase; Citrate hydro lyase; IREB1; IRP 1; Iron responsive element binding protein 1.
Background	Bifunctional iron sensor that switches between 2 activities depending on iron availability.Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization.
Application Dilution
WB	1:500-2000
IF(ICC)	IFICC1:50-200