NPC1 Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62437r-cy5-100ul |
| Product Name | NPC1 Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Niemann pick C1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4864 |
| Swiss Prot | O15118 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Niemann Pick C1 protein precursor; NPC; NPC1. |
| Background | Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment.Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | 1:50-200 |
| Flow-Cyt | 1:50-100 |
Frequently asked questions
Which applications is NPC1 Recombinant Antibody, Cy5 Conjugated (bsm-62437R-Cy5) validated for, and at what dilution?
bsm-62437R-Cy5 is validated for WB (1:300-5000); IF (1:50-200); Flow-Cyt (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NPC1 Recombinant Antibody, Cy5 Conjugated (bsm-62437R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62437R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NPC1 Recombinant Antibody, Cy5 Conjugated (bsm-62437R-Cy5)?
KLH conjugated synthetic peptide derived from human Niemann pick C1
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-62437R-Cy5)?
Cy5 on bsm-62437R-Cy5: excitation / emission 625,650nm/670nm.