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UBE2B Recombinant Antibody, FITC Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
Overview
Catalog # bsm-62449r-fitc
Product Name UBE2B Recombinant Antibody, FITC Conjugated
Applications
WB IF
Reactivity Human
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human UBE2B
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7320
Swiss Prot P63146
Subcellular location Nucleus, Cell membrane
Synonyms hHR6B; HR6B; RAD6B; UBC2; UBE2B.
Background Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1.
Application Dilution
WB 1:300-5000
IF

Frequently asked questions

Which applications is UBE2B Recombinant Antibody, FITC Conjugated (bsm-62449R-FITC) validated for, and at what dilution?

bsm-62449R-FITC is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does UBE2B Recombinant Antibody, FITC Conjugated (bsm-62449R-FITC) react with?

Tested reactivity: Human.

How should bsm-62449R-FITC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise UBE2B Recombinant Antibody, FITC Conjugated (bsm-62449R-FITC)?

KLH conjugated synthetic peptide derived from human UBE2B

What are the excitation and emission wavelengths of the FITC conjugate (bsm-62449R-FITC)?

FITC on bsm-62449R-FITC: excitation / emission 494nm/518nm.