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MED4 Recombinant Antibody, AbBy Fluor-405 Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-62459r-bf405
Product Name MED4 Recombinant Antibody, AbBy Fluor-405 Conjugated
Applications
WB IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation AbBy Fluor-405
Host Rabbit
Source KLH conjugated synthetic peptide derived from human MED4
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 29079
Swiss Prot Q9NPJ6
Subcellular location Nucleus
Synonyms ARC36; DRIP36; HSPC126; med4; TRAP36; VDRIP.
Background Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Application Dilution
WB 1:300-5000
IF

Frequently asked questions

Which applications is MED4 Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-62459R-BF405) validated for, and at what dilution?

bsm-62459R-BF405 is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does MED4 Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-62459R-BF405) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-62459R-BF405 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise MED4 Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-62459R-BF405)?

KLH conjugated synthetic peptide derived from human MED4

What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bsm-62459R-BF405)?

AbBy Fluor-405 on bsm-62459R-BF405: excitation / emission 401nm/421nm.