Acetyl-Histone H3.1 (Lys28) Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62460r-cy3 |
| Product Name | Acetyl-Histone H3.1 (Lys28) Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Histone H3.1 around the acetylation site of K28 |
| Modification Site | Lys28 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8350 |
| Swiss Prot | P68431 |
| Subcellular location | Nucleus |
| Synonyms | Histone H3.1, Histone H3, HIST1H3A. |
| Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1ug/Test |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Acetyl-Histone H3.1 (Lys28) Recombinant Antibody, Cy3 Conjugated (bsm-62460R-Cy3) validated for, and at what dilution?
bsm-62460R-Cy3 is validated for WB(WB) (1:500-2000); FCM(FCM) (1ug/Test); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Acetyl-Histone H3.1 (Lys28) Recombinant Antibody, Cy3 Conjugated (bsm-62460R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62460R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Acetyl-Histone H3.1 (Lys28) Recombinant Antibody, Cy3 Conjugated (bsm-62460R-Cy3)?
KLH conjugated synthetic peptide derived from human Histone H3.1 around the acetylation site of K28
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-62460R-Cy3)?
Cy3 on bsm-62460R-Cy3: excitation / emission 512,550nm/570,615nm.