UBE4A Recombinant Monoclonal Antibody, APC-Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62465r-apc-cy5 |
| Product Name | UBE4A Recombinant Monoclonal Antibody, APC-Cy5 Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | APC-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human UBE4A |
| Clonality | Monoclonal |
| Clone # | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9354 |
| Swiss Prot | Q14139 |
| Synonyms | UBE4A; UBOX2; UFD2; UFD2b. |
| Background | Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. Mediates 'Lys-48'-linked polyubiquitination of substrates. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is UBE4A Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-62465R-APC-Cy5) validated for, and at what dilution?
bsm-62465R-APC-Cy5 is validated for WB (1:300-5000); FCM (1:20-100); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does UBE4A Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-62465R-APC-Cy5) react with?
Tested reactivity: Human.
How should bsm-62465R-APC-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise UBE4A Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-62465R-APC-Cy5)?
KLH conjugated synthetic peptide derived from human UBE4A
What are the excitation and emission wavelengths of the APC-Cy5 conjugate (bsm-62465R-APC-Cy5)?
APC-Cy5 on bsm-62465R-APC-Cy5: excitation / emission 650nm/670nm.