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SPFH2 Recombinant Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-62469r-fitc
Product Name SPFH2 Recombinant Antibody, FITC Conjugated
Applications
WB IF(IHC-P) IF(ICC)
Reactivity Human, Mouse
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Erlin 2
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 11160
Swiss Prot O94905
Subcellular location Cytoplasm, Cell membrane
Synonyms ERLIN2; NET32; SPFH2.
Background Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs) such as ITPR1.
Application Dilution
WB 1:500-2000
IF(IHC-P) IFIHC-P1:100-500
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is SPFH2 Recombinant Antibody, FITC Conjugated (bsm-62469R-FITC) validated for, and at what dilution?

bsm-62469R-FITC is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SPFH2 Recombinant Antibody, FITC Conjugated (bsm-62469R-FITC) react with?

Tested reactivity: Human, Mouse.

How should bsm-62469R-FITC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SPFH2 Recombinant Antibody, FITC Conjugated (bsm-62469R-FITC)?

KLH conjugated synthetic peptide derived from human Erlin 2

What are the excitation and emission wavelengths of the FITC conjugate (bsm-62469R-FITC)?

FITC on bsm-62469R-FITC: excitation / emission 494nm/518nm.