PHD3 Recombinant Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62480r-bf594 |
| Product Name | PHD3 Recombinant Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PHD3 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 112399 |
| Swiss Prot | Q9H6Z9 |
| Subcellular location | Nucleus |
| Synonyms | Egl nine homolog 3; EGLN3; Factor responsive smooth muscle protein; HIF Prolyl Hydroxylase 3; HIFP4H3; HIFPH3; P4H3; PHD3; SM20. |
| Background | Prolyl hydroxylase that mediates hydroxylation of proline residues in target proteins, such as PKM, TELO2, ATF4 and HIF1A.Target proteins are preferentially recognized via a LXXLAP motif. Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is PHD3 Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-62480R-BF594) validated for, and at what dilution?
bsm-62480R-BF594 is validated for WB(WB) (1:500-2000); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PHD3 Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-62480R-BF594) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62480R-BF594 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PHD3 Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-62480R-BF594)?
KLH conjugated synthetic peptide derived from human PHD3
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bsm-62480R-BF594)?
AbBy Fluor-594 on bsm-62480R-BF594: excitation / emission 590nm/617nm.