RGMA Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62481r-rbitc-100uL |
| Product Name | RGMA Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Repulsive guidance molecule A |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 56963 |
| Swiss Prot | Q96B86 |
| Subcellular location | Cell membrane |
| Synonyms | Repulsive guidance molecule A; RGM; RGM domain family member A; RGMA. |
| Background | Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is RGMA Recombinant Antibody, RBITC Conjugated (bsm-62481R-RBITC) validated for, and at what dilution?
bsm-62481R-RBITC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RGMA Recombinant Antibody, RBITC Conjugated (bsm-62481R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62481R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RGMA Recombinant Antibody, RBITC Conjugated (bsm-62481R-RBITC)?
KLH conjugated synthetic peptide derived from human Repulsive guidance molecule A
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-62481R-RBITC)?
RBITC on bsm-62481R-RBITC: excitation / emission 570nm/595nm.