HIF-1 Alpha Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-62518r-rbitc-100uL |
| Product Name | HIF-1 Alpha Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HIF 1 alpha |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3091 |
| Swiss Prot | Q16665 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | HIF1; MOP1; PASD8; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF1A. |
| Background | Functions as a master transcriptional regulator of the adaptive response to hypoxia.Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is HIF-1 Alpha Recombinant Antibody, RBITC Conjugated (bsm-62518R-RBITC) validated for, and at what dilution?
bsm-62518R-RBITC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HIF-1 Alpha Recombinant Antibody, RBITC Conjugated (bsm-62518R-RBITC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bsm-62518R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HIF-1 Alpha Recombinant Antibody, RBITC Conjugated (bsm-62518R-RBITC)?
KLH conjugated synthetic peptide derived from human HIF 1 alpha
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-62518R-RBITC)?
RBITC on bsm-62518R-RBITC: excitation / emission 570nm/595nm.