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PPM1E Recombinant Antibody, PE-Cy5 Conjugated

Applications

  • WB
  • Flow-Cyt

Reactivity

  • Human
Overview
Catalog # bsm-62529r-pe-cy5-100uL
Product Name PPM1E Recombinant Antibody, PE-Cy5 Conjugated
Applications
WB FLOW-CYT
Reactivity Human
Specifications
Conjugation PE-Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Protein phosphatase 1E
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 22843
Swiss Prot Q8WY54
Subcellular location Cytoplasm, Nucleus
Synonyms CaMKN; POPX1; PP2CH; Ppm1e.
Background Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2.Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42. Dephosphorylates PRKAA2.
Application Dilution
WB 1:300-5000
Flow-Cyt 1ug/Test

Frequently asked questions

Which applications is PPM1E Recombinant Antibody, PE-Cy5 Conjugated (bsm-62529R-PE-Cy5) validated for, and at what dilution?

bsm-62529R-PE-Cy5 is validated for WB (1:300-5000); Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PPM1E Recombinant Antibody, PE-Cy5 Conjugated (bsm-62529R-PE-Cy5) react with?

Tested reactivity: Human.

How should bsm-62529R-PE-Cy5 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PPM1E Recombinant Antibody, PE-Cy5 Conjugated (bsm-62529R-PE-Cy5)?

KLH conjugated synthetic peptide derived from human Protein phosphatase 1E

What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bsm-62529R-PE-Cy5)?

PE-Cy5 on bsm-62529R-PE-Cy5: excitation / emission 488nm/670nm.