MYL12B Recombinant Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62539r-bf555-100uL |
| Product Name | MYL12B Recombinant Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Myosin light chain 12B |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 103910 |
| Swiss Prot | O14950 |
| Subcellular location | Cytoplasm |
| Synonyms | MLC 2A; MLC20; MRLC2; MYL12B; MYLC2B. |
| Background | Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is MYL12B Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-62539R-BF555) validated for, and at what dilution?
bsm-62539R-BF555 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MYL12B Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-62539R-BF555) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62539R-BF555 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MYL12B Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-62539R-BF555)?
KLH conjugated synthetic peptide derived from human Myosin light chain 12B
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bsm-62539R-BF555)?
AbBy Fluor-555 on bsm-62539R-BF555: excitation / emission 553nm/568nm.