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KLK8 Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with KLK8 monoclonal antibody, unconjugated (bsm-62551R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with KLK8 monoclonal antibody, unconjugated (bsm-62551R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with KLK8 Monoclonal Antibody, Unconjugated (bsm-62551R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with KLK8 Monoclonal Antibody, Unconjugated (bsm-62551R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with KLK8 Monoclonal Antibody, Unconjugated (bsm-62551R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with KLK8 Monoclonal Antibody, Unconjugated (bsm-62551R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with KLK8 Monoclonal Antibody, Unconjugated (bsm-62551R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with KLK8 Monoclonal Antibody, Unconjugated (bsm-62551R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Left Parietal Lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with KLK8 Monoclonal Antibody, Unconjugated (bsm-62551R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Left Parietal Lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with KLK8 Monoclonal Antibody, Unconjugated (bsm-62551R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF(IHC-P)

Reactivity

  • Human
Overview
Catalog # bsm-62551r
Product Name KLK8 Recombinant Antibody
Applications
WB IHC-P IHC-F IF(IHC-P)
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Kallikrein 8
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 11202
Swiss Prot O60259
Subcellular location Cytoplasm, Secreted
Synonyms BSP1; hK8; HNP; HPN; Kallikrein8; Klk8; NRPN; PRSS19; TADG14; UNQ283.
Background Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity.
Application Dilution
WB 1:500-2000
IHC-P IHC-P1:100-500
IHC-F IHC-F1:100-500
IF(IHC-P) IFIHC-P1:100-500

Frequently asked questions

Which applications is KLK8 Recombinant Antibody (bsm-62551R) validated for, and at what dilution?

bsm-62551R is validated for WB(WB) (1:500-2000); IHC-P(IHC-P) (1:100-500); IHC-F(IHC-F) (1:100-500); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does KLK8 Recombinant Antibody (bsm-62551R) react with?

Tested reactivity: Human.

How should bsm-62551R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise KLK8 Recombinant Antibody (bsm-62551R)?

KLH conjugated synthetic peptide derived from human Kallikrein 8

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