Help

HARS Recombinant Antibody, AbBy Fluor-488 Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
Overview
Catalog # bsm-62552r-bf488-100uL
Product Name HARS Recombinant Antibody, AbBy Fluor-488 Conjugated
Applications
WB IF
Reactivity Human
Specifications
Conjugation AbBy Fluor-488
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Histidine tRNA ligase
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3035
Swiss Prot P12081
Subcellular location Cytoplasm
Synonyms HARS; HisRS; histidine translase; HRS; USH3B.
Background Catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate. Plays a role in axon guidance.
Application Dilution
WB 1:300-5000
IF

Frequently asked questions

Which applications is HARS Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-62552R-BF488) validated for, and at what dilution?

bsm-62552R-BF488 is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HARS Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-62552R-BF488) react with?

Tested reactivity: Human.

How should bsm-62552R-BF488 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HARS Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-62552R-BF488)?

KLH conjugated synthetic peptide derived from human Histidine tRNA ligase

What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bsm-62552R-BF488)?

AbBy Fluor-488 on bsm-62552R-BF488: excitation / emission 499nm/519nm.