PNMT Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with PNMT monoclonal antibody, unconjugated (bsm-62565R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with PNMT monoclonal antibody, unconjugated (bsm-62565R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
ICC/IF

Reactivity

Human
Mouse

Overview
Catalog #	bsm-62565r
Product Name	PNMT Recombinant Antibody
Applications	WB ICC/IF
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PNMTase
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5409
Swiss Prot	P11086
Subcellular location	Cytoplasm
Synonyms	PENT; PNMT; PNMTase.
Background	Catalyzes the transmethylation of nonepinephrine (noradrenaline) to form epinephrine (adrenaline), using S-adenosyl-L-methionine as the methyl donor. Other substrates include phenylethanolamine and octopamine. Also methylates normetanephrine.
Application Dilution
WB	1:300-5000
ICC/IF	IFICC1:50-200