Nck beta Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62597r-cy5-100uL |
| Product Name | Nck beta Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NCK2 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8440 |
| Swiss Prot | O43639 |
| Subcellular location | Cytoplasm |
| Synonyms | GRB4; NCK adaptor protein 2; NCK2; NCKbeta. |
| Background | Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is Nck beta Recombinant Antibody, Cy5 Conjugated (bsm-62597R-Cy5) validated for, and at what dilution?
bsm-62597R-Cy5 is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Nck beta Recombinant Antibody, Cy5 Conjugated (bsm-62597R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62597R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Nck beta Recombinant Antibody, Cy5 Conjugated (bsm-62597R-Cy5)?
KLH conjugated synthetic peptide derived from human NCK2
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-62597R-Cy5)?
Cy5 on bsm-62597R-Cy5: excitation / emission 625,650nm/670nm.