BLNK Recombinant Antibody, Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62608r-cy7-100uL |
| Product Name | BLNK Recombinant Antibody, Cy7 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human BLNK |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 29760 |
| Swiss Prot | Q8WV28 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | AGM4; BASH; Bca; BLNK; Ly57; Lyw57; SLP65. |
| Background | Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes. |
| Application Dilution | |
| FCM | 1:50-100 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:100-500 |
Frequently asked questions
Which applications is BLNK Recombinant Antibody, Cy7 Conjugated (bsm-62608R-Cy7) validated for, and at what dilution?
bsm-62608R-Cy7 is validated for FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does BLNK Recombinant Antibody, Cy7 Conjugated (bsm-62608R-Cy7) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62608R-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise BLNK Recombinant Antibody, Cy7 Conjugated (bsm-62608R-Cy7)?
KLH conjugated synthetic peptide derived from human BLNK
What are the excitation and emission wavelengths of the Cy7 conjugate (bsm-62608R-Cy7)?
Cy7 on bsm-62608R-Cy7: excitation / emission 743nm/767nm.