Help

SARA Recombinant Antibody, RBITC Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
Overview
Catalog # bsm-62621r-rbitc-100uL
Product Name SARA Recombinant Antibody, RBITC Conjugated
Applications
WB IF
Reactivity Human
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SARA
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9372
Swiss Prot O95405
Subcellular location Cytoplasm
Synonyms hSARA; MADHIP; NSP; SAR1; SARA; SARA1; SMADIP; ZFYVE9.
Background Early endosomal protein that functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. Plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex.
Application Dilution
WB 1:300-5000
IF

Frequently asked questions

Which applications is SARA Recombinant Antibody, RBITC Conjugated (bsm-62621R-RBITC) validated for, and at what dilution?

bsm-62621R-RBITC is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SARA Recombinant Antibody, RBITC Conjugated (bsm-62621R-RBITC) react with?

Tested reactivity: Human.

How should bsm-62621R-RBITC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SARA Recombinant Antibody, RBITC Conjugated (bsm-62621R-RBITC)?

KLH conjugated synthetic peptide derived from human SARA

What are the excitation and emission wavelengths of the RBITC conjugate (bsm-62621R-RBITC)?

RBITC on bsm-62621R-RBITC: excitation / emission 570nm/595nm.