UBA3 Recombinant Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62623r-apc-100uL |
| Product Name | UBA3 Recombinant Antibody, APC Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human UBA3 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9039 |
| Swiss Prot | Q8TBC4 |
| Synonyms | hUba3; NEDD8 activating enzyme E1C; uba3; UBE1C. |
| Background | Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is UBA3 Recombinant Antibody, APC Conjugated (bsm-62623R-APC) validated for, and at what dilution?
bsm-62623R-APC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does UBA3 Recombinant Antibody, APC Conjugated (bsm-62623R-APC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62623R-APC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise UBA3 Recombinant Antibody, APC Conjugated (bsm-62623R-APC)?
KLH conjugated synthetic peptide derived from human UBA3
What are the excitation and emission wavelengths of the APC conjugate (bsm-62623R-APC)?
APC on bsm-62623R-APC: excitation / emission 650nm/660nm.