BCKDH kinase Recombinant Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62631r-Cy5.5-100uL |
| Product Name | BCKDH kinase Recombinant Antibody, Cy5.5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human BCKDH kinase |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10295 |
| Swiss Prot | O14874 |
| Synonyms | BCKD kinase; BCKDHKIN; Bckdk; BCKDKD; BDK. |
| Background | Serine/threonine-protein kinase component of macronutrients metabolism. Forms a functional kinase and phosphatase pair with PPM1K, serving as a metabolic regulatory node that coordinates branched-chain amino acids (BCAAs) with glucose and lipid metabolism via two distinct phosphoprotein targets: mitochondrial BCKDHA subunit of the branched-chain alpha-ketoacid dehydrogenase (BCKDH) complex and cytosolic ACLY, a lipogenic enzyme of Krebs cycle. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is BCKDH kinase Recombinant Antibody, Cy5.5 Conjugated (BSM-62631R-CY5-5) validated for, and at what dilution?
BSM-62631R-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does BCKDH kinase Recombinant Antibody, Cy5.5 Conjugated (BSM-62631R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BSM-62631R-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise BCKDH kinase Recombinant Antibody, Cy5.5 Conjugated (BSM-62631R-CY5-5)?
KLH conjugated synthetic peptide derived from human BCKDH kinase
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BSM-62631R-CY5-5)?
Cy5.5 on BSM-62631R-CY5-5: excitation / emission 675nm/694nm.