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Phospho-EIF4B (Ser406) Recombinant Antibody, AbBy Fluor-680 Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-62634r-bf680-100uL
Product Name Phospho-EIF4B (Ser406) Recombinant Antibody, AbBy Fluor-680 Conjugated
Applications
WB IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation AbBy Fluor-680
Host Rabbit
Source KLH conjugated synthetic peptide derived from human eIF4B around the phosphorylation site of S406
Modification Site Ser406
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1975
Swiss Prot P23588
Subcellular location Cytoplasm
Synonyms EIF4B
Background Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Application Dilution
WB 1:300-5000
IF

Frequently asked questions

Which applications is Phospho-EIF4B (Ser406) Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-62634R-BF680) validated for, and at what dilution?

bsm-62634R-BF680 is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Phospho-EIF4B (Ser406) Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-62634R-BF680) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-62634R-BF680 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Phospho-EIF4B (Ser406) Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-62634R-BF680)?

KLH conjugated synthetic peptide derived from human eIF4B around the phosphorylation site of S406

What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bsm-62634R-BF680)?

AbBy Fluor-680 on bsm-62634R-BF680: excitation / emission 679nm/702nm.