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Phospho-EIF4B (Ser406) Recombinant Antibody, Biotin Conjugated

Applications

  • WB
  • IHC-P
  • IHC-F

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-62634r-biotin-100uL
Product Name Phospho-EIF4B (Ser406) Recombinant Antibody, Biotin Conjugated
Applications
WB IHC-P IHC-F
Reactivity Human, Mouse, Rat
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from human eIF4B around the phosphorylation site of S406
Modification Site Ser406
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 1975
Swiss Prot P23588
Subcellular location Cytoplasm
Synonyms EIF4B
Background Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is Phospho-EIF4B (Ser406) Recombinant Antibody, Biotin Conjugated (bsm-62634R-Biotin) validated for, and at what dilution?

bsm-62634R-Biotin is validated for WB (1:300-5000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Phospho-EIF4B (Ser406) Recombinant Antibody, Biotin Conjugated (bsm-62634R-Biotin) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-62634R-Biotin be stored?

Store at -20°C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Phospho-EIF4B (Ser406) Recombinant Antibody, Biotin Conjugated (bsm-62634R-Biotin)?

KLH conjugated synthetic peptide derived from human eIF4B around the phosphorylation site of S406