Phospho-EIF4B (Ser406) Recombinant Antibody, PE Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62634r-pe-100uL |
| Product Name | Phospho-EIF4B (Ser406) Recombinant Antibody, PE Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human eIF4B around the phosphorylation site of S406 |
| Modification Site | Ser406 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1975 |
| Swiss Prot | P23588 |
| Subcellular location | Cytoplasm |
| Synonyms | EIF4B |
| Background | Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is Phospho-EIF4B (Ser406) Recombinant Antibody, PE Conjugated (bsm-62634R-PE) validated for, and at what dilution?
bsm-62634R-PE is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-EIF4B (Ser406) Recombinant Antibody, PE Conjugated (bsm-62634R-PE) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62634R-PE be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-EIF4B (Ser406) Recombinant Antibody, PE Conjugated (bsm-62634R-PE)?
KLH conjugated synthetic peptide derived from human eIF4B around the phosphorylation site of S406
What are the excitation and emission wavelengths of the PE conjugate (bsm-62634R-PE)?
PE on bsm-62634R-PE: excitation / emission 496,564nm/578nm.