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RNF14 Recombinant Antibody, AbBy Fluor-350 Conjugated

Applications

  • WB

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-62639r-bf350-100uL
Product Name RNF14 Recombinant Antibody, AbBy Fluor-350 Conjugated
Applications
WB
Reactivity Human, Mouse, Rat
Specifications
Conjugation AbBy Fluor-350
Host Rabbit
Source KLH conjugated synthetic peptide derived from human RNF14
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9604
Swiss Prot Q9UBS8
Subcellular location Cytoplasm, Nucleus
Synonyms ARA54; HFB30; Rnf14; TRIAD2.
Background E3 ubiquitin-protein ligase that plays a key role in the RNF14-RNF25 translation quality control pathway, a pathway that takes place when a ribosome has stalled during translation, and which promotes ubiquitination and degradation of translation factors on stalled ribosomes.Recruited to stalled ribosomes by the ribosome collision sensor GCN1 and mediates 'Lys-6'-linked ubiquitination of target proteins, leading to their degradation.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is RNF14 Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-62639R-BF350) validated for, and at what dilution?

bsm-62639R-BF350 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does RNF14 Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-62639R-BF350) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-62639R-BF350 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise RNF14 Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-62639R-BF350)?

KLH conjugated synthetic peptide derived from human RNF14

What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bsm-62639R-BF350)?

AbBy Fluor-350 on bsm-62639R-BF350: excitation / emission 346nm/442nm.