RNF14 Recombinant Antibody, PE Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62639r-pe-100uL |
| Product Name | RNF14 Recombinant Antibody, PE Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RNF14 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9604 |
| Swiss Prot | Q9UBS8 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | ARA54; HFB30; Rnf14; TRIAD2. |
| Background | E3 ubiquitin-protein ligase that plays a key role in the RNF14-RNF25 translation quality control pathway, a pathway that takes place when a ribosome has stalled during translation, and which promotes ubiquitination and degradation of translation factors on stalled ribosomes.Recruited to stalled ribosomes by the ribosome collision sensor GCN1 and mediates 'Lys-6'-linked ubiquitination of target proteins, leading to their degradation. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is RNF14 Recombinant Antibody, PE Conjugated (bsm-62639R-PE) validated for, and at what dilution?
bsm-62639R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RNF14 Recombinant Antibody, PE Conjugated (bsm-62639R-PE) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62639R-PE be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RNF14 Recombinant Antibody, PE Conjugated (bsm-62639R-PE)?
KLH conjugated synthetic peptide derived from human RNF14
What are the excitation and emission wavelengths of the PE conjugate (bsm-62639R-PE)?
PE on bsm-62639R-PE: excitation / emission 496,564nm/578nm.