Phospho-MDM2 (Ser166) Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62661r-cy5-100uL |
| Product Name | Phospho-MDM2 (Ser166) Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MDM2 around the phosphorylation site of S166 |
| Modification Site | Ser166 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4193 |
| Swiss Prot | Q00987 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | ACTFS; Hdm2; HDMX; MDM2; MDM2BP; MTBP. |
| Background | E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-100 |
Frequently asked questions
Which applications is Phospho-MDM2 (Ser166) Recombinant Antibody, Cy5 Conjugated (bsm-62661R-Cy5) validated for, and at what dilution?
bsm-62661R-Cy5 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-MDM2 (Ser166) Recombinant Antibody, Cy5 Conjugated (bsm-62661R-Cy5) react with?
Tested reactivity: Human.
How should bsm-62661R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-MDM2 (Ser166) Recombinant Antibody, Cy5 Conjugated (bsm-62661R-Cy5)?
KLH conjugated synthetic peptide derived from human MDM2 around the phosphorylation site of S166
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-62661R-Cy5)?
Cy5 on bsm-62661R-Cy5: excitation / emission 625,650nm/670nm.