SAFB Recombinant Antibody

$Western blot analysis of 293 cell lysate. Using SAFB (bsm-62719R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of 293 cell lysate. Using SAFB (bsm-62719R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
IHC-P
IHC-F
IF
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-62719r
Product Name	SAFB Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IF, ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human SAF B
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6294
Swiss Prot	Q15424
Synonyms	HAP; HET; SAFB; SAFB1.
Background	Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing.Functions as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF	ICC1:50-200
ICC/IF	IF