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Phospho-Rb2 p130 (T986) Recombinant Antibody, AbBy Fluor-594 Conjugated

Applications

  • WB

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-62720r-bf594-100uL
Product Name Phospho-Rb2 p130 (T986) Recombinant Antibody, AbBy Fluor-594 Conjugated
Applications
WB
Reactivity Human, Mouse
Specifications
Conjugation AbBy Fluor-594
Host Rabbit
Source KLH conjugated synthetic peptide derived from human RBL2 around the phosphorylation site of T986
Modification Site Thr986
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5934
Swiss Prot Q08999
Subcellular location Nucleus
Synonyms 130 kDa retinoblastoma associated protein; IB4; p130; PRB2; Rb2; Rbl2; RBR2; Retinoblastoma like 2; Retinoblastoma-related protein 2; RIC128.
Background Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is Phospho-Rb2 p130 (T986) Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-62720R-BF594) validated for, and at what dilution?

bsm-62720R-BF594 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Phospho-Rb2 p130 (T986) Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-62720R-BF594) react with?

Tested reactivity: Human, Mouse.

How should bsm-62720R-BF594 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Phospho-Rb2 p130 (T986) Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-62720R-BF594)?

KLH conjugated synthetic peptide derived from human RBL2 around the phosphorylation site of T986

What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bsm-62720R-BF594)?

AbBy Fluor-594 on bsm-62720R-BF594: excitation / emission 590nm/617nm.