UFC1 Recombinant Antibody

$Western blot analysis of A549 cell lysate. Using UFC1 (bsm-62735R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of A549 cell lysate. Using UFC1 (bsm-62735R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
IHC-P
IHC-F
IP
IF
ICC/IF

Reactivity

Human

Overview
Catalog #	bsm-62735r
Product Name	UFC1 Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IP, IF, ICC/IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human UFC1
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	51506
Swiss Prot	Q9Y3C8
Subcellular location	Extracellular, Secreted
Synonyms	HSPC155; Ubiquitin fold modifier conjugating enzyme 1; UFC1; Ufm1 conjugating enzyme 1.
Background	E1-like enzyme which specifically catalyzes the second step in ufmylation.Accepts the ubiquitin-like modifier UFM1 from the E1 enzyme UBA5 and forms an intermediate with UFM1 via a thioester linkage.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IP	1-2ug
IF
ICC/IF	1:50-100