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NOLC1 Recombinant Antibody, PE-Cy7 Conjugated

Applications

  • WB

Reactivity

  • Human
Overview
Catalog # bsm-62739r-pe-cy7
Product Name NOLC1 Recombinant Antibody, PE-Cy7 Conjugated
Applications
WB
Reactivity Human
Specifications
Conjugation PE-Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NOLC1
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9221
Swiss Prot Q14978
Subcellular location Cytoplasm, Nucleus
Synonyms NOLC1; NOPP130; Nopp140; NS5ATP13; Nucleolar phosphoprotein p130; P130.
Background Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification.Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is NOLC1 Recombinant Antibody, PE-Cy7 Conjugated (bsm-62739R-PE-Cy7) validated for, and at what dilution?

bsm-62739R-PE-Cy7 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NOLC1 Recombinant Antibody, PE-Cy7 Conjugated (bsm-62739R-PE-Cy7) react with?

Tested reactivity: Human.

How should bsm-62739R-PE-Cy7 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NOLC1 Recombinant Antibody, PE-Cy7 Conjugated (bsm-62739R-PE-Cy7)?

KLH conjugated synthetic peptide derived from human NOLC1

What are the excitation and emission wavelengths of the PE-Cy7 conjugate (bsm-62739R-PE-Cy7)?

PE-Cy7 on bsm-62739R-PE-Cy7: excitation / emission 488nm/780nm.