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c-Jun & JunD (phospho-S73/S100) Recombinant Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-62758r-fitc
Product Name c-Jun & JunD (phospho-S73/S100) Recombinant Antibody, FITC Conjugated
Applications
WB IF(IHC-P)
Reactivity Human, Mouse
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human c Jun around the phosphorylation site of S73
Modification Site Ser73+Ser100
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3725
Swiss Prot P05412,P17535
Subcellular location Nucleus
Synonyms JunD; c-Jun.
Background Transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3'. Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to the AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing its transcriptional activity.
Application Dilution
WB 1:500-2000
IF(IHC-P) IFIHC-P1:50-200

Frequently asked questions

Which applications is c-Jun & JunD (phospho-S73/S100) Recombinant Antibody, FITC Conjugated (bsm-62758R-FITC) validated for, and at what dilution?

bsm-62758R-FITC is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does c-Jun & JunD (phospho-S73/S100) Recombinant Antibody, FITC Conjugated (bsm-62758R-FITC) react with?

Tested reactivity: Human, Mouse.

How should bsm-62758R-FITC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise c-Jun & JunD (phospho-S73/S100) Recombinant Antibody, FITC Conjugated (bsm-62758R-FITC)?

KLH conjugated synthetic peptide derived from human c Jun around the phosphorylation site of S73

What are the excitation and emission wavelengths of the FITC conjugate (bsm-62758R-FITC)?

FITC on bsm-62758R-FITC: excitation / emission 494nm/518nm.