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ATE1 Recombinant Antibody, RBITC Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-62759r-rbitc
Product Name ATE1 Recombinant Antibody, RBITC Conjugated
Applications
WB IF(IHC-P)
Reactivity Human, Mouse, Rat
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Arginyltransferase 1
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 11101
Swiss Prot O95260
Subcellular location Cytoplasm, Nucleus
Synonyms Arginyltransferase 1; Ate1; R transferase 1.
Background Involved in the post-translational conjugation of arginine to the N-terminal aspartate or glutamate of a protein. This arginylation is required for degradation of the protein via the ubiquitin pathway. Does not arginylate cysteine residues.
Application Dilution
WB 1:500-2000
IF(IHC-P) IFIHC-P1:100-500

Frequently asked questions

Which applications is ATE1 Recombinant Antibody, RBITC Conjugated (bsm-62759R-RBITC) validated for, and at what dilution?

bsm-62759R-RBITC is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ATE1 Recombinant Antibody, RBITC Conjugated (bsm-62759R-RBITC) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-62759R-RBITC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ATE1 Recombinant Antibody, RBITC Conjugated (bsm-62759R-RBITC)?

KLH conjugated synthetic peptide derived from human Arginyltransferase 1

What are the excitation and emission wavelengths of the RBITC conjugate (bsm-62759R-RBITC)?

RBITC on bsm-62759R-RBITC: excitation / emission 570nm/595nm.