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IKK alpha/beta Recombinant Antibody

Western blot analysis of (1) A431 cell lysate; (2) 293T cell lysate; (3) Mouse kidney lysate. Using IKK alpha\/beta (bsm-62764R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n
Western blot analysis of (1) A431 cell lysate; (2) 293T cell lysate; (3) Mouse kidney lysate. Using IKK alpha\/beta (bsm-62764R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-IKK alpha\/beta antibody (bsm-62764R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-IKK alpha\/beta antibody (bsm-62764R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (IKK alpha\/beta) monoclonal Antibody, unconjugated (bsm-62764R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (IKK alpha\/beta) monoclonal Antibody, unconjugated (bsm-62764R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Bioss unconjugated antibody vial

Applications

  • WB
  • IP
  • ICC/IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-62764r
Product Name IKK alpha/beta Recombinant Antibody
Applications
WB IP ICC/IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human IKK alpha
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 3551
Swiss Prot O14920
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms I-kappa-B kinase 2; I-kappa-B-kinase beta; IKBKB; IKK-B; IKK-beta; IKK2; IKKB.
Background Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses.
Application Dilution
WB 1:300-5000
IP IFICC1:50-200
ICC/IF IP1-2ug

Frequently asked questions

Which applications is IKK alpha/beta Recombinant Antibody (bsm-62764R) validated for, and at what dilution?

bsm-62764R is validated for WB (1:300-5000); IF(ICC) (1:50-200); IP (1-2ug). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does IKK alpha/beta Recombinant Antibody (bsm-62764R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-62764R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise IKK alpha/beta Recombinant Antibody (bsm-62764R)?

KLH conjugated synthetic peptide derived from human IKK alpha

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