Help

eIF3A Recombinant Antibody, Cy3 Conjugated

Applications

  • WB
  • IF
  • Flow-Cyt

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-62797r-cy3
Product Name eIF3A Recombinant Antibody, Cy3 Conjugated
Applications
WB IF FLOW-CYT
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human eIF3A
Clonality Recombinant
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 8661
Swiss Prot Q14152
Subcellular location Cytoplasm
Synonyms EIF3; eIF3 theta; eIF3a; EIF3S10; P167; p180; p185; TIF32.
Background Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis.
Application Dilution
WB 1:500-2000
IF 1:100-500
Flow-Cyt 1:100-200

Frequently asked questions

Which applications is eIF3A Recombinant Antibody, Cy3 Conjugated (bsm-62797R-Cy3) validated for, and at what dilution?

bsm-62797R-Cy3 is validated for WB (1:500-2000); IF (1:100-500); Flow-Cyt (1:100-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does eIF3A Recombinant Antibody, Cy3 Conjugated (bsm-62797R-Cy3) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-62797R-Cy3 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise eIF3A Recombinant Antibody, Cy3 Conjugated (bsm-62797R-Cy3)?

KLH conjugated synthetic peptide derived from human eIF3A

What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-62797R-Cy3)?

Cy3 on bsm-62797R-Cy3: excitation / emission 512,550nm/570,615nm.