p45 NF-E2 Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62800r-cy5 |
| Product Name | p45 NF-E2 Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NFE2 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4778 |
| Swiss Prot | Q16621 |
| Subcellular location | Nucleus |
| Synonyms | nfe2 ; Nuclear factor; p45 NFE2. |
| Background | Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is p45 NF-E2 Recombinant Antibody, Cy5 Conjugated (bsm-62800R-Cy5) validated for, and at what dilution?
bsm-62800R-Cy5 is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does p45 NF-E2 Recombinant Antibody, Cy5 Conjugated (bsm-62800R-Cy5) react with?
Tested reactivity: Human.
How should bsm-62800R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise p45 NF-E2 Recombinant Antibody, Cy5 Conjugated (bsm-62800R-Cy5)?
KLH conjugated synthetic peptide derived from human NFE2
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-62800R-Cy5)?
Cy5 on bsm-62800R-Cy5: excitation / emission 625,650nm/670nm.